Nuclear & Organoid Segmentation, volume & fluorescence intensity quantification scripts

Author

Affiliation

Richard Butler

The Gurdon Institute

Abstract

Segment nuclei in DAPI channel excluding aggregates and measure intensity in each channel. Segment an organoid in the DAPI channel, measure proportion of volume positive for signal in C2, C3, C4 and C2+C3

Files

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DOI:10.5281/zenodo.14627141

Papers

The emergence of human primordial germ cell–like cells in stem cell–derived gastruloids
DOI:10.1126/sciadv.ado1350