{"id":["DOI:10.1126/sciadv.ado1350"],"title":["The emergence of human primordial germ cell–like cells in stem cell–derived gastruloids"],"publication_date":["2025-03-26"],"authors":{"ORCID:0000-0001-7848-2431":{"id":["ORCID:0000-0001-7848-2431"],"family_name":["Neupane"],"personal_name":["Jitesh"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]},"ROR:013meh722":{"organization_name":["University of Cambridge"]}},"HDBItheme":["Early"]},"ORCID:0009-0007-7732-3974":{"id":["ORCID:0009-0007-7732-3974"],"family_name":["Lubatti"],"personal_name":["Gabriele"],"affiliations":{"ROR:00cfam450":{"organization_name":["Helmholtz Zentrum München"]}}},"ORCID:0000-0003-3300-713X":{"id":["ORCID:0000-0003-3300-713X"],"family_name":["Gross-Thebing"],"personal_name":["Theresa"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]},"ROR:013meh722":{"organization_name":["University of Cambridge"]}}},"ORCID:0000-0002-3885-1332":{"id":["ORCID:0000-0002-3885-1332"],"family_name":["Butler"],"personal_name":["Richard"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}},"ORCID:0000-0003-4217-4458":{"id":["ORCID:0000-0003-4217-4458"],"family_name":["Ruiz Tejada Segura"],"personal_name":["Mayra Luisa"],"affiliations":{"ROR:00cfam450":{"organization_name":["Helmholtz Zentrum München"]}}},"ORCID:0000-0002-0204-3405":{"id":["ORCID:0000-0002-0204-3405"],"family_name":["Gross-Thebing"],"personal_name":["Sargon"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}},"ORCID:0000-0001-6672-8457":{"id":["ORCID:0000-0001-6672-8457"],"family_name":["Dietmann"],"personal_name":["Sabine"],"affiliations":{"ROR:01yc7t268":{"organization_name":["Washington University in St. Louis"]}}},"ORCID:0000-0002-4956-2843":{"id":["ORCID:0000-0002-4956-2843"],"family_name":["Scialdone"],"personal_name":["Antonio"],"affiliations":{"ROR:00cfam450":{"organization_name":["Helmholtz Zentrum München"]}}},"ORCID:0000-0002-8640-4318":{"id":["ORCID:0000-0002-8640-4318"],"family_name":["Surani"],"personal_name":["Azim"],"affiliations":{"ROR:013meh722":{"organization_name":["University of Cambridge"]},"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}},"HDBItheme":["Early"]}},"abstract":["Most advances in early human postimplantation development depend on animal studies and stem cell-based embryo models. Here, we present self-organized three-dimensional human gastruloids (hGs) derived from embryonic stem cells. The transcriptome profile of day 3 hGs aligned with Carnegie stage 7 human gastrula, with cell types and differentiation trajectories consistent with human gastrulation. Notably, we observed the emergence of nascent primordial germ cell-like cells (PGCLCs), but without exogenous bone morphogenetic protein (BMP) signaling, which is essential for the PGCLC fate. A mutation in the ISL1 gene affects amnion-like cells and leads to a loss of PGCLCs; the addition of exogenous BMP2 rescues the PGCLC fate, indicating that the amnion may provide endogenous BMP signaling. Our model of early human embryogenesis will enable further exploration of the germ line and other early human lineages."],"HDBItheme":["Early"],"datasets":{"ArrayExpress:E-MTAB-12045":{"id":["ArrayExpress:E-MTAB-12045"],"title":["Self-organisation of human pluripotent stem cells into three-dimensional model of post-implantation development"],"description":["Self-organisation of human pluripotent stem cells into three-dimensional model of post-implantation development, termed as embryonic organoids (hEO), recapitulates crucial features of human gastrulation and early neurulation. Transcriptional characterisation of hEO at different stages of development to study origin and specification of human primordial germ cells (PGCs), emergence of neuromesodermal progenitors (NMP), establishment of primary germ layers and their derivatives and appearance of neuronal precursors."],"authors":{"ORCID:0009-0007-7732-3974":{"id":["ORCID:0009-0007-7732-3974"],"family_name":["Lubatti"],"personal_name":["Gabriele"],"affiliations":{"ROR:00cfam450":{"organization_name":["Helmholtz Zentrum München"]}}}},"tags":["sequencing"]}},"code":{"DOI:10.5281/zenodo.14627141":{"id":["DOI:10.5281/zenodo.14627141"],"title":["Nuclear & Organoid Segmentation, volume & fluorescence intensity quantification scripts"],"description":["Segment nuclei in DAPI channel excluding aggregates and measure intensity in each channel.\nSegment an organoid in the DAPI channel, measure proportion of volume positive for signal in C2, C3, C4 and C2+C3"],"authors":{"ORCID:0000-0002-3885-1332":{"id":["ORCID:0000-0002-3885-1332"],"family_name":["Butler"],"personal_name":["Richard"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}}}}}}