{"id":["DOI:10.1016/j.celrep.2025.116373"],"title":["A post-implantation model of human embryo development includes a definitive hematopoietic niche"],"publication_date":["2025-10-28"],"authors":{"ORCID:0000-0001-7848-2431":{"id":["ORCID:0000-0001-7848-2431"],"family_name":["Neupane"],"personal_name":["Jitesh"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]},"ROR:013meh722":{"organization_name":["University of Cambridge"]}},"HDBItheme":["Early"]},"ORCID:0000-0002-8436-6637":{"id":["ORCID:0000-0002-8436-6637"],"family_name":["Jowett"],"personal_name":["Geraldine M"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]},"ROR:013meh722":{"organization_name":["University of Cambridge"]}}},"ORCID:0000-0002-8224-6885":{"id":["ORCID:0000-0002-8224-6885"],"family_name":["Wu"],"personal_name":["Baojiang"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]},"ROR:0106qb496":{"organization_name":["Inner Mongolia University"]}}},"ORCID:0000-0002-9791-4997":{"id":["ORCID:0000-0002-9791-4997"],"family_name":["Verma"],"personal_name":["Ankit"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}},"ORCID:0000-0001-6672-8457":{"id":["ORCID:0000-0001-6672-8457"],"family_name":["Dietmann"],"personal_name":["Sabine"],"affiliations":{"ROR:01yc7t268":{"organization_name":["Washington University in St. Louis"]}}},"ORCID:0000-0002-5926-7768":{"id":["ORCID:0000-0002-5926-7768"],"family_name":["Reid"],"personal_name":["Adam James"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}},"ORCID:0000-0002-8640-4318":{"id":["ORCID:0000-0002-8640-4318"],"family_name":["Surani"],"personal_name":["Azim"],"affiliations":{"ROR:013meh722":{"organization_name":["University of Cambridge"]},"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}},"HDBItheme":["Early"]}},"abstract":["Stem cell-derived embryo models are crucial for investigations to advance our knowledge of early human development. Here, we present a post-gastrulation three-dimensional (3D) embryo model that is kinetically matured to promote multi-lineage organogenesis with tissues comparable to those found in Carnegie stage (CS)12-CS16 human embryos. The resulting structures include cardiomyocytes, hepatocytes, endothelial cells, and hematopoietic cells, but they lack a yolk sac. Notably, we observe SOX17+RUNX1+ hemogenic buds, where we detect the maturation of hematopoietic stem cells (HSCs). These hemogenic niches, where endothelial-to-hematopoietic transition occurs, contain instructive (DLL4, SCF) and restrictive (FGF23) factors for the maturation of HSC. These HSCs have the potential to differentiate into myeloid and lymphoid lineages, and, therefore, they are equivalent to definitive hematopoiesis. Accordingly, we call our model hematoids, which offer both a versatile tool for investigating tissue-scale mechanisms of human development and a potential source of human HSCs for mechanistic studies and cell therapies."],"HDBItheme":["Early"],"datasets":{"ArrayExpress:E-MTAB-13632":{"id":["ArrayExpress:E-MTAB-13632"],"title":["Single-cell RNA-seq of a three-dimensional multilineage human organogenesis model showing endothelial-to-hematopoietic-transition in vitro"],"description":["Multilineage differentiation of human embryonic stem cells into three-dimensional (3-D) model, termed as progressed elongated embryoid bodies (peEBs), recapitulates crucial features of early human development, including cardio myogenesis and haematopoiesis. Characterisation of peEBs at transcriptome and protein levels at  different stages of development to study embryonic haematopoiesis and  endothelial-to-hematopoietic-transition (EHT) in vitro."],"authors":{"ORCID:0000-0002-5926-7768":{"id":["ORCID:0000-0002-5926-7768"],"family_name":["Reid"],"personal_name":["Adam James"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}}},"tags":["sequencing"]}},"code":{"SWHID:swh:1:dir:23e10a60da6a5f9e84417e64204716bc6fb962b1":{"id":["SWHID:swh:1:dir:23e10a60da6a5f9e84417e64204716bc6fb962b1"],"title":["Neupane et al. Hematoids"],"description":["R code invovled in processing, analysing and generating figures from single-cell RNA-seq data associated with Neupane et al. - \"A post-implantation embryo model of human development with a definitive hematopoietic niche\"\nThe sample names used here are not used in the paper, the relationship is:\n\n    heo_E1 - SB-late day 8\n    heo_F1 - SB-late day 14,\n    heo_H10 - SB-early day 8\n    heo_G1 - SB-early day 14\n\nThe primary data and the *.rds files of containing Seurat objects for each sample are available from Array Express"],"authors":{"ORCID:0000-0002-5926-7768":{"id":["ORCID:0000-0002-5926-7768"],"family_name":["Reid"],"personal_name":["Adam James"],"affiliations":{"ROR:00fp3ce15":{"organization_name":["The Gurdon Institute"]}}}}}}}