{"id":["DOI:10.1016/j.cell.2021.04.013"],"title":["Parental genome unification is highly error-prone in mammalian embryos"],"publication_date":["2021-05-27"],"authors":{"ORCID:0000-0003-2544-5340":{"id":["ORCID:0000-0003-2544-5340"],"family_name":["Cavazza"],"personal_name":["Tommaso"],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}},"ORCID:0000-0002-8401-624X":{"id":["ORCID:0000-0002-8401-624X"],"family_name":["Takeda"],"personal_name":["Yuko"],"affiliations":{"ROR:03myjph80":{"organization_name":["Wellcome Centre for Mitochondrial Research"]}},"HDBItheme":["Early"]},"ORCID:0000-0003-4788-0933":{"id":["ORCID:0000-0003-4788-0933"],"family_name":["Politi"],"personal_name":["Antonio Z."],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}},"ORCID:0000-0002-1348-6404":{"id":["ORCID:0000-0002-1348-6404"],"family_name":["Aushev"],"personal_name":["Magomet"],"affiliations":{"ROR:03myjph80":{"organization_name":["Wellcome Centre for Mitochondrial Research"]}},"HDBItheme":["Early"]},"AUTHTEMP:00013":{"id":["AUTHTEMP:00013"],"family_name":["Aldag"],"personal_name":["Patrick"],"affiliations":{"ROR:025fw7a54":{"organization_name":["Friedrich-Loeffler-Institut"]}}},"AUTHTEMP:00014":{"id":["AUTHTEMP:00014"],"family_name":["Baker"],"personal_name":["Clara"],"affiliations":{"ROR:04qg15997":{"organization_name":["Bourn Hall Clinic"]}}},"ORCID:0000-0003-0483-3627":{"id":["ORCID:0000-0003-0483-3627"],"family_name":["Choudhary"],"personal_name":["Meenakshi"],"affiliations":{"ROR:05p40t847":{"organization_name":["Newcastle upon Tyne Hospitals NHS Foundation Trust"]}}},"ORCID:0000-0001-5725-8940":{"id":["ORCID:0000-0001-5725-8940"],"family_name":["Bucevičius"],"personal_name":["Jonas"],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}},"ORCID:0000-0002-7176-1793":{"id":["ORCID:0000-0002-7176-1793"],"family_name":["Lukinavičius"],"personal_name":["Gražvydas"],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}},"ORCID:0000-0003-3510-8268":{"id":["ORCID:0000-0003-3510-8268"],"family_name":["Elder"],"personal_name":["Kay"],"affiliations":{"ROR:04qg15997":{"organization_name":["Bourn Hall Clinic"]}}},"AUTHTEMP:00015":{"id":["AUTHTEMP:00015"],"family_name":["Blayney"],"personal_name":["Martyn"],"affiliations":{"ROR:04qg15997":{"organization_name":["Bourn Hall Clinic"]}}},"ORCID:0000-0003-0438-7444":{"id":["ORCID:0000-0003-0438-7444"],"family_name":["Lucas-Hahn"],"personal_name":["Andrea"],"affiliations":{"ROR:025fw7a54":{"organization_name":["Friedrich-Loeffler-Institut"]}}},"AUTHTEMP:00016":{"id":["AUTHTEMP:00016"],"family_name":["Niemann"],"personal_name":["Heiner"],"affiliations":{"ROR:025fw7a54":{"organization_name":["Friedrich-Loeffler-Institut"]},"ROR:00f2yqf98":{"organization_name":["Hannover Medical School"]}}},"ORCID:0000-0002-8488-2770":{"id":["ORCID:0000-0002-8488-2770"],"family_name":["Herbert"],"personal_name":["Mary"],"affiliations":{"ROR:03myjph80":{"organization_name":["Wellcome Centre for Mitochondrial Research"]},"ROR:05p40t847":{"organization_name":["Newcastle upon Tyne Hospitals NHS Foundation Trust"]}},"HDBItheme":["Early"]},"ORCID:0000-0003-0025-8952":{"id":["ORCID:0000-0003-0025-8952"],"family_name":["Schuh"],"personal_name":["Melina"],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}}},"abstract":["Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to develop into healthy euploid embryos. Here, we show that the parental genomes cluster with nucleoli in each pronucleus within human and bovine zygotes, and clustering is required for the reliable unification of the parental genomes after fertilization. During migration of intact pronuclei, the parental genomes polarize toward each other in a process driven by centrosomes, dynein, microtubules, and nuclear pore complexes. The maternal and paternal chromosomes eventually cluster at the pronuclear interface, in direct proximity to each other, yet separated. Parental genome clustering ensures the rapid unification of the parental genomes on nuclear envelope breakdown. However, clustering often fails, leading to chromosome segregation errors and micronuclei, incompatible with healthy embryo development."],"HDBItheme":["Early"],"code":{"SWHID:swh:1:dir:99b72c30ebb767297a70fa7296e748f77ed7232f":{"id":["SWHID:swh:1:dir:99b72c30ebb767297a70fa7296e748f77ed7232f"],"title":["Compute nuclear chromatin distribution in zygotes"],"description":["The workflow computes metrics of the chromatin distribution in the two nuclei of a zygote before nuclear envelope breakdown. The metrics are\n- Inner/outer fractions: Quantify chromatin intensities in oriented sections of the nucleus. The sections are oriented in the direction of the 2 nuclei. - Maximal polarity: Find an orientation along each nuclei that maximizes the chromatin fraction in one of the sections. - Nuclear occupancy: Quantifies the amount of chromatin with respect to the total volume of the nucleus. - Surface occupancy: Quantifies chromatin intensity in a region close to the surface with respect to inside of the nucleus.\nThe workflow uses different software and plugins. In short, starting from a .czi file the user has to\n1. Prepare data for processing (IJ script SplitTpointsH2bchannel.ijm) 2. Manually specify nuclei masks using spheres (IJ script BoneJFitSphere.ijm) 3. Compute the maximal polarity (IJ plugin nuclei polarity) 4. Segment and compute the distribution of the chromatin mass (Matlab code computePoiDistribution.m)_"],"authors":{"ORCID:0000-0003-4788-0933":{"id":["ORCID:0000-0003-4788-0933"],"family_name":["Politi"],"personal_name":["Antonio Z."],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}}}}},"reagents":{"URL:https://www.addgene.org/browse/article/28216708/":{"id":["URL:https://www.addgene.org/browse/article/28216708/"],"title":["Parental genome unification is highly error-prone in mammalian embryos"],"description":["171497\tpGEMHE-mClover3\tT7 promotor drives in vitro transcription of mClover3 mRNA\n171496\tpGEMHE-mH33B-mClover3\tT7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA\n171495\tpGEMHE-bNup98-DN-NLS-mClover3\tT7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA\n171494\tpGEMHE-mClover3-bElys\tT7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA\n171493\tpGEMHE-GST-KASH5-DN\tT7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA\n171492\tpGEMHE-mClover3-KASH5-DN\tT7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA\n171491\tpGEMHE-Pom121-mScarlet\tT7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA\n171490\tpGEMHE-mScarlet-hCenpC\tT7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA\n171489\tpGEMHE-H2B-mClover3\tT7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA\n171488\tpGEMHE-mScarlet-MAP4-MTBD\tT7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA\n171487\tpGEMHE-miRFP-MAP4-MTBD\tT7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA\n171486\tpGEMHE-GST\tT7 promotor drives in vitro transcription of GST mRNA"],"authors":{"ORCID:0000-0003-0025-8952":{"id":["ORCID:0000-0003-0025-8952"],"family_name":["Schuh"],"personal_name":["Melina"],"affiliations":{"ROR:03av75f26":{"organization_name":["Max Planck Institute for Multidisciplinary Sciences"]}}}}}}}