{"id":["DOI:10.6084/m9.figshare.28597145"],"title":["Suppression of ERK signalling promotes pluripotent epiblast in the human blastocyst"],"description":["Confocal microscopy images (.lif) of human embryos from the manuscript: Simon et. al., (2025) Suppression of ERK signalling promotes pluripotent epiblast in the human blastocyst.\nFGF experiments (related to Fig. 1a):\n\n    Experiments 220318, 220512, 220520, 220705, 241203, and 241223\n    Control vs FGF (250ng/ml FGF4 + Heparin, 500ng/ml FGF4 + Heparin, and 750ng/ml FGF4 + Heparin) treated Day 5 human embryos for 36 hours.\n    Immunofluorescence staining for GATA4 (A488, hypoblast), NANOG (A594, epiblast), and GATA3 (A647, trophectoderm) and counterstained with DAPI.\n    Imaged on Leica SP8 Scanning Confocal Microscopy.\n\npERK experiments (related to Fig. 2a):\n\n    Experiments 211021, 211105 and 220120\n    Control Day 5, Day 6 and Day 6.5 human embryos\n    Immunofluorescence staining for SOX2 (A488, epiblast), pERK (A594) and OTX2 (A647, hypoblast) and counterstained with DAPI.\n    Imaged on Leica SP8 Scanning Confocal Microscopy.\n\nERKi experiments (related to Fig. 2d and 4a):\n\n    Experiments 220205, 220225, and 220311\n    Control (0.1% DMSO) vs ERKi (5μM Ulixertinib) treated Day 5 human embryos for 36 hours.\n    Immunofluorescence staining for GATA4 (A488, hypoblast), NANOG (A594, epiblast), and GATA3 (A647, trophectoderm) and counterstained with DAPI.\n    Imaged on Leica SP8 Scanning Confocal Microscopy.\n\n    Experiment 231212\n    Control (0.1% DMSO) vs ERKi (5μM Ulixertinib) treated Day 5 human embryos for 36 hours.\n    Immunofluorescence staining for KLF17 (A488 - naive pluripotency), KLF4 (A555, failed), and SOX2 (A647, epiblast) and counterstained with DAPI.\n    Imaged on Leica SP8 Scanning Confocal Microscopy.\n\nPlease see manuscript for detailed materials and methods\nDownstream quantification of fluorescence intensities performed with an image analysis pipeline available on GitLab: https://gitlab.developers.cam.ac.uk/ctr/ctr-bioinformatics/niakan-lab/simon-et-al-2025"],"authors":{"ORCID:0000-0001-9614-1403":{"id":["ORCID:0000-0001-9614-1403"],"family_name":["Simon"],"personal_name":["Claire S."],"affiliations":{"ROR:013meh722":{"organization_name":["University of Cambridge"]}}}}}